Beverage-Air NC49 Manual

AminoPac® PA10 AAA-DirectTM

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 9 of 66SECTION 2 - OPERATION AND SYSTEM REQUIREMENTS2.1 System Require

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 10 of 662.2 System Operation RequirementsThe Dionex AAA-Direct Amino A

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 11 of 66SECTION 3 - PURITY REQUIREMENTS FOR CHEMICALSObtaining reliabl

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 12 of 66SECTION 4 - GETTING STARTED4.1 The Most Important RulesALWAYS

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 13 of 664.2 Initial Check ListThese items MUST be available in your la

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 14 of 66SECTION 5 - PREPARATION OF ELUENTS AND STANDARDSNOTEAlways san

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 15 of 665.3 Eluent E3: 1.0 M Sodium AcetateA. Using 18.2 megohm-cm wat

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 16 of 66SECTION 6 - SYSTEM INSTALLATION AND START-UPThere are four dis

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 17 of 66E. When completed, you should not see any tubing sticking up a

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 18 of 666.3 Verification of Column Cleanliness(Optional: If installing

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 1 of 66PRODUCT MANUALfor theAMINOPAC™ PA10 ANALYTICAL COLUMN(2 x 250 m

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 19 of 666.4 Verification of System Response6.4.1 Adjusting the Eluent

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 20 of 666.5 Verification of System Functionality6.5.1 Injection of NIS

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 21 of 666.6 System ShutdownAs with all amino acid analyses, the best r

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 22 of 66SECTION 7 - SELECTING DETECTION AND GRADIENT METHODS7.1 Introd

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 23 of 66CAUTIONNever leave a reference electrode inside a disconnected

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 24 of 667.2 AminoPac PA10 Test ChromatogramFigure 5, “AminoPac PA10 Te

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 25 of 667.3 Selection of Gradient MethodTable 2Overview of Gradient Me

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 26 of 66Converting from Post-Separation Cleanup to On-the-Fly CleanupP

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 27 of 66Table 2BFast Gradient Conditions, AutomatedPlease note that th

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 28 of 66Table 2CGradient Conditions - Manual, Discontinuous Operation7

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 2 of 66TABLE OF CONTENTSSECTION 1 - INTRODUCTION ...

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 29 of 66SECTION 8 - APPLICATIONSThe detection waveform in Table 1 has

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 30 of 668.1 Simultaneous Monitoring of Amino Acids And Carbohydrates i

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 31 of 668.2 Analysis of Amino Acids in HydrolysatesThe present techniq

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 32 of 668.3 Analysis of TryptophanUnder certain conditions, it is poss

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 33 of 66543211098761413121116150 5.00 10.00 15.00 20.00 25.00 30.00Min

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 34 of 668.5 Influence of Temperature on the Separation of Amino Acids

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 35 of 668.6 Analysis of Phospho-Amino AcidsPhospho amino acids, being

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 36 of 668.7 MSA Hydrolysis of Meat SamplesIn this section, samples wer

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 37 of 668.8 Free Amino Acids in Beverage SamplesThe gradient condition

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 38 of 661. Arginine2. Hydroxylysine3. Lysine4. Galactosamine5. Glucosa

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 3 of 66SECTION 6 - SYSTEM INSTALLATION AND START-UP ...

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 39 of 668.9 Amino Acids and Sugars in a Cell Culture MediaFigure 16A s

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 40 of 668.10 AAA Recommended Reading1. Clarke, A. P., Jandik, P., Rock

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 41 of 6617. Ding, Y., Hong, Y., and Mou, S. “Direct Determination of F

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 42 of 66SECTION 9 – SPECIALIZED GRADIENT METHODSThe initial selection

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 43 of 669.2 Gradient Conditions for Protein HydrolysatesA good startin

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 44 of 669.3 Gradient Conditions Including a Strong Post-Separation Rin

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 45 of 669.4 Gradient Conditions for an Improved Peak Shape of Histidin

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 46 of 669.5 Gradient Method for Improved Long-Term System StabilityThe

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 47 of 669.6 Gradient Method for Complex Mixtures of Amino Acids and Ca

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 48 of 66SECTION 10 - TROUBLESHOOTINGKeep in mind that some of the prob

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 4 of 66SECTION 9 – SPECIALIZED GRADIENT METHODS ...

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 49 of 66The affected gold working electrode P/N 55832 can be reconditi

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 50 of 66C. Verify that a shortest possible length of 0.010" i.d.

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 51 of 660 5 10 15 20 25 30 35 40 456080100120140nC12345678910111213141

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 52 of 6610.4.4Incorrect or Variable Retention TimesA. Check your eluen

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 53 of 66NOTEMake sure that all internal surfaces (for example: entire

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 54 of 66M. Connect the system pump, injector valve, yellow tubing and

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 55 of 66M. Run a sequence of several 25 µL injections of a 8 µM histid

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 56 of 6610.8.3Chemical Reconditioning of AAA Gold Working ElectrodesTh

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 57 of 66SECTION 11 - IPAD POSITIVE COMPOUNDS SEPARATED ON AMINOPAC PA1

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 58 of 66Glucuronic acid 22.46 Phenylalanine 1 194.14Glutamate 23.46 14

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 5 of 66SECTION 13 - INSTALLATION OF REFERENCE ELECTRODE ...

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 59 of 66Compound RT (Min) Coelution Resolving MW pKapKbpKxS with Meth

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 60 of 66SECTION 12 - INSTALLATION OF DISPOSABLE ELECTRODECAUTIONRead a

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 61 of 66The Following Waveforms Must be Used With Disposable Electrode

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 62 of 66SECTION 13 - INSTALLATION OF REFERENCE ELECTRODEWhat has chang

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 63 of 66Step 4Verify that you have all of the following parts:NOTEThe

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 64 of 66Step 7Carefully insert the reference electrode into the refere

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 65 of 66APPENDIX A - COLUMN CAREA.1 New Column EquilibrationThe column

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 66 of 66a flat surface such as a razor blade. Make sure the end of the

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 6 of 66SECTION 1 - INTRODUCTION1.1 Dionex AAA-DirectThe Dionex AAA-Dir

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 7 of 66NOTEThroughout this manual, we discuss the 80 nC Rule for non-d

Document No. 031481-12 AAA-Direct, Dionex Amino Analyzer and AminoPac PA10 Page 8 of 661.5 Replacement Parts for AAA-Certified Electrochemical Gold Ce