Beverage-Air NC49 manuals

Beverage air NC49 Manual (67 pages)

Brand: Beverage-air | Category: Unknown | Size: 2.31 MB |

English

Table of contents

AminoPac

1

AAA-Direct

1

©Dionex Corporation, 2006

2

Document No. 031481

2

Revision 12

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November 2006

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TABLE OF CONTENTS

3

SECTION 1 - INTRODUCTION

7

2.1 System Requirements

10

3.1 Deionized Water

12

3.2 Sodium Hydroxide

12

3.3 Sodium Acetate

12

SECTION 4 - GETTING STARTED

13

4.2 Initial Check List

14

5.5 Amino Acid Standards

16

6.1.2 Software Installation

18

6.1.3 System Rinse

18

6.2.1 System Background Check

18

Ca. 30 nC

19

6.4.2 Column Installation

20

6.4.3 Histidine Injection

20

Overlay of lines 3 and 4

21

Peak height ~150 nC

21

6.6 System Shutdown

22

Figure 5

25

Overview of Gradient Methods

26

SECTION 8 - APPLICATIONS

30

HCl Hydrolysate

32

M Standard

32

8.3 Analysis of Tryptophan

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Standard

34

Performic Acid/HCl

34

Hydrolysate

34

0 5.00 10.00 15.00 20.00

35

Carrot Juice

39

Figure 16B

40

Figure 16A

40

8.10 AAA Recommended Reading

41

Eluent 1: 10 mM NaOH

47

Eluent 2: 250 mM NaOH

47

Gradient Conditions for

48

above gradient table

48

SECTION 10 - TROUBLESHOOTING

49

10.3 Column Problems

50

10.4 System Problems

51

Oxygen Dip

52

10.5 Sodium Hydroxide Cleanup

53

10.6 Nitric Acid Cleanup

54

10.7 Acetate Line Cleanup

55

10.8.1 Mechanical Polishing

56

Reference Electrode

63

Cylinder

63

Thumbscrew

63

Cell Cover

63

Cell Body

63

J2 Connector

64

Junction

65

APPENDIX A - COLUMN CARE

66

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